Goal of Dynamo


The goal of project DYNAMO is an in-depth understanding of the biogenesis of energy-transducing membranes by integrating knowledge from research devoted to the regulation of gene expression, to structural and membrane biology and to bioenergetics. The project focuses on three major areas through three distinct but complementary tasks:

Task 1: Gene expression from bacteria to organelles

Task 1 of DYNAMO addresses the evolution of post-transcriptional regulation from bacteria, where all aspects of the cell's metabolism are under the control of its own gene expression machinery, to organelles, where these evolutionarily conserved tools primarily drive the expression of membrane proteins under the hierarchical control of nuclear-encoded factors. Consequently this task aims at comparing mRNA decay, translation initiation and the global post-transcriptional regulation in bacteria and chloroplast as well as evaluating a potential role for non-coding RNAs in the chloroplast.

Task 2: Membrane biogenesis and dynamics

Task 2 of DYNAMO focuses on the regulation of membrane proliferation and dynamics and its cross-talk with that of protein expression. For these purposes, biogenesis and membrane proliferation are monitored in two model systems: The C. reinhardtii chloroplast and E. coli bacteria. In parallel, dynamics of bacteria, chloroplasts and mitochondria is explored through study of a family of ancestral large GTPases that modulate fission and fusion of lipid bilayers, the Dynamin Related Proteins (DRPs), with particular emphasis on their implication in membrane fusion.

Task 3: Supramolecular organization of membrane proteins and membranes

Task 3 of DYNAMO is devoted to the structural and functional changes in protein-protein interactions that occur upon assembly into an active complex and its derived supercomplexes, that shape the supramolecular organization of these membranes and that have profound functional consequences that are particular to bioenergetic membranes. In this context, this task aims at resolving the high resolution structures of the b6f complex from Chlamydomonas reinhardtii and the Rieske/cytb complex of Bacillus subtilis and focuses on the importance of Quinones in bioenergetic membranes.

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